导读：生物方面的文献翻译，求助大神帮忙，要求语句通顺即可。 生物方面的文献翻译，求助大神帮忙，要求语句通顺即可。Genetic diversity among microorganisms has been widely studied by PCR-based techniques, which have opened avenues for the development of...
生物方面的文献翻译，求助大神帮忙，要求语句通顺即可。
生物方面的文献翻译，求助大神帮忙，要求语句通顺即可。
Genetic diversity among microorganisms has been widely studied by PCR-based techniques, which have opened avenues for the development of new and inventive molecular typing methods . Genotypic relationships among microorganisms have been determined by analyzing the genomic DNA with PCR-based methods . Repetitive sequence-based PCR (Rep-PCR) has been used successfully to generate DNA fingerprints to distinguish between genetically unrelated isolates and closely related bacterial strains . It involves the use of primers based on the short repetitive elements derived from highly conserved palindromic inverted repeat regions dispersed throughout the prokaryotic kingdom . Amplification of the regions between adjacent repetitive extragenic elements gives strain-specific DNA fingerprints . Primer sets based on the repetitive elements present in the bacterial genome, ERIC (enterobacterial repetitive intergenic consensus),REP (repetitive extragenic palindromes), and BOX, yield genomic fingerprints specific to pathovars and strains of gramnegative bacteria . The inter-REP and inter-ERIC profiles are specific for bacterial strains within a species .
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基于 PCR 技术，已开辟发展的新的创造性分子分型方法的途径，其广泛研究了微生物的遗传多样性。通过对基因组 DNA 分析与基于 PCR 的方法确定了微生物之间的遗传关系。重复序列基于 PCR （Rep-PCR） 已被成功地用于生成区分基因无关的菌株和密切相关的细菌菌株的 DNA 指纹。它涉及使用的引物的基础派生从分散在原核生物王国的高度保守复发倒立重复区域短的重复元素。扩增的重复序列片段基因的相邻...
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