谁帮我把这段文章翻译成英文,翻译好的多给分!

谁帮我把这段文章翻译成英文,翻译好的多给分!
采用Sanger双脱氧链末端终止法,按T7 Sequencing TM Kit使用说明操作.[n一 P]
dATP标记.8% 变性聚丙烯酰胺凝胶,厚度0．4mm,胶长55cm,电泳在Pharmach LKB公司的Macrophor测序仪上进行,1200V~1500V,2～5小时.测序产物置x
光负片爆光,于一20℃过夜,放射自显影.
AT PJtts基固克隆与表达 将pPGAP —AT用NcoI—SalI双酶切,插入pBV220中,得到pBV220一a1一AT重组质粒.再将其中的a1-AT(BamHl—Ava1)片段用PCR定点诱变后的突变片段(BamHI—AvaI)置换,即得到垒基目的pBV220 AT P[tts表达质粒.该
质粒转化大肠杆菌HB101,在LB培养基中30"C生长至对数中期．42"C诱导3小时.收集菌体,超声破碎后分别用1mol／L,2mol／L尿素洗涤,8mol/L尿素溶解
包涵体. 密接关系 1年前他留下的回答 已收到6个回答

rrnh123 网友

该名网友总共回答了16个问题，此问答他的回答如下：采纳率：87.5%

比较靠谱的翻译：
采用Sanger双脱氧链末端终止法,按T7 Sequencing TM Kit使用说明操作.[n一 P] dATP标记.8% 变性聚丙烯酰胺凝胶,厚度0．4mm,胶长55cm,电泳在Pharmach LKB公司的Macrophor测序仪上进行,1200V~1500V,5小时.测序产物置x光负片爆光,于一20℃过夜,放射自显影.
Sanger’s dideoxynucleotide chain terminating method was adopted and conducted according to the application instructions of T7 Sequencing TM Kit.Marked as [n一 P] dATP.The electrophoresis of 8%denaturing polyacrylamide gel with 0.4mm thickness and 55cm in length was conducted on Pharmach LKB Company’s Macrophor DNA sequencing machine at 1200V~1500V for 5 hours.The sequenced product was then subject to X-ray negative film explosion light and autoradiography for overnight at - 20°C.
AT PJtts基固克隆与表达:将pPGAP —AT用NcoI—SalI双酶切,插入pBV220中,得到pBV220一a1一AT重组质粒.再将其中的a1-AT(BamHl—Ava1)片段用PCR定点诱变后的突变片段(BamHI—AvaI)置换,即得到垒基目的pBV220 AT P[tts表达质粒.
AT PJtts gene cloning and expression:NcoI—SalI double enzyme digestion was conducted on pPGAP —AT,and it was inserted into pBV220 vector to obtain pBV220一a1一AT recombinant plasmid.One of the a1-AT(BamHl—Ava1) gene fragments was displaced by (BamHI—Ava1) which was a mutated fragment from PCR site directed mutagenesis process,and thus barrier based purpose pBV220 AT P[tts expression plasmids were obtained.
该质粒转化大肠杆菌HB101,在LB培养基中30"C生长至对数中期．42"C诱导3小时.收集菌体,超声破碎后分别用1mol／L,2mol／L尿素洗涤,8mol/L尿素溶解
包涵体.
The plasmids were transformed into Escherichia coli HB101,they were cultured in LB medium at 30"C until mid-logarithmic growth and induced for 3 hours at 42"C.The thalli were collected and subject to ultrasonication; they were separately cleansed by using 1mol／L and 2mol／L urea,and then dissolved in 8 mol/L urea.
【英语牛人团】

1年前他留下的回答

2

一苇之所如 网友

该名网友总共回答了4个问题，此问答他的回答如下：

Using Sanger dideoxy chain termination method using T7 Sequencing TM Kit according to the instructions. [N a P]
dATP labeled. 8% polyacrylamide gel, the thickness of 0.4mm, plastic long 55cm, ele...

1年前他留下的回答

2

hhy0312 网友

该名网友总共回答了13个问题，此问答他的回答如下：

采用Sanger双脱氧链末端终止法，按T7 Sequencing TM Kit使用说明操作。[n一 P]
Using Sanger dideoxy chain termination method, according to the T7 Sequencing TM Kit usage instructions. [ n P]
dATP标记。8% 变性聚丙烯酰胺凝胶，厚度0．4mm...

1年前他留下的回答

2

tianlengxixi 网友

该名网友总共回答了2个问题，此问答他的回答如下：

翻译
The Sanger double deoxidizing chain end, according to terminate method T7 Sequencing TM Kit instructions operation. [n a P]
DATP mark. 8% degeneration polyacrylamide gel, thickness of 0.4 m...

1年前他留下的回答

1

欧洲 网友

该名网友总共回答了2个问题，此问答他的回答如下：

Using Sanger dideoxy chain termination method, according to the T7 Sequencing TM Kit usage instructions. [ n P]DATP markers. 8% denaturing polyacrylamide gel, gel thickness 0.4mm, long 55cm, electroph...

1年前他留下的回答

0

高雅的丰采 网友

该名网友总共回答了42个问题，此问答他的回答如下：

The Sanger double deoxidizing chain end, according to terminate method T7 Sequencing TM Kit instructions operation. [n a P]
DATP mark. 8% degeneration polyacrylamide gel, thickness of 0.4 mm, 55 c...

1年前他留下的回答

0

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